Regulation of neurofibromin expression in rat sciatic nerve and cultured Schwann cells
Identifieur interne : 000930 ( Main/Exploration ); précédent : 000929; suivant : 000931Regulation of neurofibromin expression in rat sciatic nerve and cultured Schwann cells
Auteurs : Lawrence Wrabetz [États-Unis, Italie] ; Maria Laura Feltri [États-Unis, Italie] ; Haesun Kim [États-Unis] ; Maryellen Daston [États-Unis] ; John Kamholz [États-Unis] ; Steven S. Scherer [États-Unis] ; Nancy Ratner [États-Unis]Source :
- Glia [ 0894-1491 ] ; 1995-09.
English descriptors
- Teeft :
- Asbury, Axon, Axotomy, Cdna, Cultured schwann cells, Daston, Declue, Degeneration, Distal stump, Distal stumps, Embryonic, Embryonic brain membrane, Final concentration, Forskolin, Gapdh, Histone, Lemke, Mrna, Mrna expression, Mrna levels, Mutation, Myelin, Myelin ovoids, Myelinating schwann cells, Nature genet, Nerve, Nerve transection, Neurofibroma, Neurofibromatosis, Neurofibromatosis gene, Neurofibromin, Neurofibromin expression, Neurofibromin levels, Ngfr, Normal schwann cells, Northern analysis, Other tissues, Peripheral nerve, Proliferation, Radiolabeled cdna probes, Ratner, Schwann, Schwann cell proliferation, Schwann cells, Sciatic, Sciatic nerve, Sciatic nerves, Transection, Wallerian, Wallerian degeneration, Western blot analysis, Wrabetz.
Abstract
Loss of function mutations at the NF1 locus may act intrinsically in Schwann cells to cause the formation of benign Schwann cell tumors (neurofibromas) in patients with type 1 neurofibromatosis. To identify contexts in Schwann cells in which such mutations may play an important role, we measured the levels of NF1 mRNA and neurofibromin in rat sciatic nerve during development, after axotomy, and in cultured rat Schwann cells. NF1 mRNA was present in developing sciatic nerve throughout the period of active Schwann cell proliferation and myelination. After nerve transection, no alteration in NF1 message level was detected, but neurofibromin levels increased, as assessed by immunohistochemistry and Western blotting, suggesting that, in vivo, neurofibromin expression in Schwann cells is post‐transcriptionally induced during Wallerian degeneration. Cultured rat Schwann cells constitutively expressed NF1 mRNA and neurofibromin. Schwann cell proliferation induced by exposure to serum and forskolin was not associated with changes in NF1 mRNA or neurofibromin expression, whereas Schwann cell proliferation induced by extracts of embryonic brain membranes was associated with increased NF1 message and neurofibromin expression. Thus, Schwann cells, both in vivo and in vitro, express NF1 mRNA constitutively; the expression of NF1 mRNA and neurofibromin is modulated by only some mitogenic stimuli in Schwann cells. © 1995 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/glia.440150104
Affiliations:
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To identify contexts in Schwann cells in which such mutations may play an important role, we measured the levels of NF1 mRNA and neurofibromin in rat sciatic nerve during development, after axotomy, and in cultured rat Schwann cells. NF1 mRNA was present in developing sciatic nerve throughout the period of active Schwann cell proliferation and myelination. After nerve transection, no alteration in NF1 message level was detected, but neurofibromin levels increased, as assessed by immunohistochemistry and Western blotting, suggesting that, in vivo, neurofibromin expression in Schwann cells is post‐transcriptionally induced during Wallerian degeneration. Cultured rat Schwann cells constitutively expressed NF1 mRNA and neurofibromin. Schwann cell proliferation induced by exposure to serum and forskolin was not associated with changes in NF1 mRNA or neurofibromin expression, whereas Schwann cell proliferation induced by extracts of embryonic brain membranes was associated with increased NF1 message and neurofibromin expression. Thus, Schwann cells, both in vivo and in vitro, express NF1 mRNA constitutively; the expression of NF1 mRNA and neurofibromin is modulated by only some mitogenic stimuli in Schwann cells. © 1995 Wiley‐Liss, Inc.</div></front></TEI><affiliations><list><country><li>Italie</li><li>États-Unis</li></country><region><li>Californie</li><li>Ohio</li><li>Pennsylvanie</li></region></list><tree><country name="États-Unis"><region name="Pennsylvanie"><name sortKey="Wrabetz, Lawrence" sort="Wrabetz, Lawrence" uniqKey="Wrabetz L" first="Lawrence" last="Wrabetz">Lawrence Wrabetz</name></region><name sortKey="Daston, Maryellen" sort="Daston, Maryellen" uniqKey="Daston M" first="Maryellen" last="Daston">Maryellen Daston</name><name sortKey="Daston, Maryellen" sort="Daston, Maryellen" uniqKey="Daston M" first="Maryellen" last="Daston">Maryellen Daston</name><name sortKey="Feltri, Maria Laura" sort="Feltri, Maria Laura" uniqKey="Feltri M" first="Maria Laura" last="Feltri">Maria Laura Feltri</name><name sortKey="Kamholz, John" sort="Kamholz, John" uniqKey="Kamholz J" first="John" last="Kamholz">John Kamholz</name><name sortKey="Kim, Haesun" sort="Kim, Haesun" uniqKey="Kim H" first="Haesun" last="Kim">Haesun Kim</name><name sortKey="Ratner, Nancy" sort="Ratner, Nancy" uniqKey="Ratner N" first="Nancy" last="Ratner">Nancy Ratner</name><name sortKey="Scherer, Steven S" sort="Scherer, Steven S" uniqKey="Scherer S" first="Steven S." last="Scherer">Steven S. Scherer</name></country><country name="Italie"><noRegion><name sortKey="Wrabetz, Lawrence" sort="Wrabetz, Lawrence" uniqKey="Wrabetz L" first="Lawrence" last="Wrabetz">Lawrence Wrabetz</name></noRegion><name sortKey="Feltri, Maria Laura" sort="Feltri, Maria Laura" uniqKey="Feltri M" first="Maria Laura" last="Feltri">Maria Laura Feltri</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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